48种中药材的乙酰胆碱酯酶抑制活性研究

摘要

Inhibition of acetylcholinesterase (AChE) is one of the approaches for the treatment of Alzheimer's disease (AD).In this paper the AChE inhibitory activities of ethanolic and aqueous extracts of 48 traditional Chinese medicinal herbs were evaluated.These traditional Chinese medicines have intelligence-promoting,anti-insomnia,sedative,neurotonic or tonic effects.Microplate assay indicated that ethanolic extracts of 28 traditional Chinese medicinal herbs and water extracts of 11 herbs showed AChE inhibitory activities.Among them,ethanolic extracts of Herba Moslae,Fructus Alpiniae Oxyphyllae,Radix Rehmanniae and Folium Nelumbinis showed most potent AChE inhibitory activities at the concentration of 0.1 mg/mL,with (68.63± 1.12)％,(44.49±3.66)％,(43.78±4.76)％,and (42.63±8.31)％ inhibition,respectively.The AChE inhibitory activities were also confirmed by TLC bioautographic assay.These results partially validate the traditional uses of some medicinal herbs for cognitive improvement.%乙酰胆碱酯酶(AChE)抑制剂是治疗阿尔茨海默病的一个重要策略.本研究对48种中药材的乙醇提取物和水提取物进行了体外AChE抑制活性的筛选,微板测试法结果表明28种中药材的乙醇提取物和1 1种中药材的水提取物具有AChE抑制活性.其中,香薷、益智仁、生地黄和荷叶乙醇提取物的AChE抑制活性最强,它们在终浓度为0.1 mg/mL时的抑制活性分别为(68.63±1.12)％,(44.49±3.66)％,(43.78±4.76)％和(42.63±8.31)％.薄层色谱-生物自显影的方法亦证明了这四种中药材具有抑制AChE的活性成分.本实验结果为治疗阿尔茨海默病中药的传统应用提供了部分支持.