16种EPA-PAHs复合污染土壤的菌群修复

摘要

通过富集筛选获得一组PAHs降解混合菌群和3株降解单菌,利用变性梯度凝胶电泳(DGGE)技术分析混合菌群的组成,对16种多环芳烃(PAHs)复合污染土壤进行生物修复,同时考察混合菌群和单菌株在PAHs复合污染土壤中的生物修复效果.结果表明:混合菌群主要由3株已分离获得的降解单菌和5株未可分离培养的单菌组成;经过30 d的生物修复,混合菌群对土壤中总PAHs的降解率(54.17％)高于单一菌株(28.40％,31.95％,24.64％),并且对高相对分子质量PAHs的降解表现出了较大的优势,4环、5环、6环PAHs的降解率分别可达到71.26％、39.76％和42.86％；利用混合菌群来修复16种PAHs复合污染的土壤,可以避免一些未可分离培养的关键菌株的丢失,使PAHs的降解更加全面有效.%For bioremediation of polycyclic aromatic hydrocarbons ( PAHs) combined contaminated-soil, a microbial consortium and three strains were isolated from PAHs contaminated-soil containing sixteen US Environmental Protection Agency priority control PAHs. Denaturing gradient gel electrophoresis (DGGE) was used to analyze the structure of the microbial consortium , and the biodegradation effect of PAHs mixtures with the inoculation of mixed microbial consortium and single strain was studied. The results show that the consortium is mainly composed of three isolated strains and five uncultured bacterias. After 30 days bioremediation, the degradation efficiency of total PAHs by microbial consortium (54. 17% ) is higher than any single strain's (28. 40% ,31. 95% ,24. 64% ). Microbial consortium shows a great advantage in degradation of high relative molecular mass PAHs, and the degradation efficiencies of four-rings, five-rings and six-rings PAHs are 71. 26% , 39. 76% , 42. 86% , respectively. Microbial consortium is capable of degrading PAHs which can avoid the loss of key strains and has a good application prospect in the bioremediation of PAHs contaminated-soil.