Comparison of the Two Chromosome Forms of Paragonimus Westermani Using DNA Restriction Fragment Size and DNA Hybridization

摘要

Restriction enzyme digestion of total genomic DNA of two chro-mosome type Paragonimus westermani showed the presence of homologus highlyrepeated DNA in diploid and triploid forms. Southern blot analysis providedfurther evidence that the distribution of restriction enzyme sites (with 3enzymes) on repeated DNA of both was similar. But with Pst Ⅰ、Dde Ⅰ、Hae Ⅲand Hpa Ⅱ. They revealed polymorphism which also occured individually ineach form tested separatedly with hybridization technique. Mutation of recog-nize sites by enzymes, which is resulted from the alternation of gene sequance,has occured during evolution. Additionally, it is probably possible, based on PstⅠand Dde Ⅰdigest pattern to distinguish between diploid and triploid Parag-onimus westermani and group the Paragonimus simply and rapidly.