Propagation of Low Temperature-Adapted Hepatitis A Virus in BSC-1 Cell Culture

摘要

Hepatitis A virus, HM-175 strain, adapted to low temperature was cultivated in BSC-1 cell culture.Replication of the virus was detected by radioimmunoassay(RIA), radioimmunofocus assay (RIFA) and dot blot hybridization. The results showed that, of the 7 low temperature-adapted(LTA) strains studied, strain E, F and D gave the highest titer in BSC-1 cell culture. This culture system was proved to be suitable for the study of the characteristic of temperature mutants of HAV.