目的 建立同时测定四逆汤中乌头碱、新乌头碱和次乌头碱含量的HPLC法.方法采用HPLC色谱法,Eclipse XDBC18色谱柱(4.6 mm×150mm,5μm),流动相甲醇-0.1％三乙胺(65:35),流速为1.0mL·min-1,柱温为30℃,检测波长为240 nm.结果乌头碱、新乌头碱、次乌头碱分别在0.120 4～6.02 μg(r =0.9997)、0.041 2～2.06 μg(r =0.999 5)、0.080 8～4.04 μg(r=0.999 9)范围内与峰面积呈良好的线性关系；平均回收率分别为100.74％( RSD=1.88％)、99.9％( RSD=2.98％)、102.9％( RSD=1.32％).结论该方法简便可行、重现性好、可作为四逆汤的质量控制方法.%Abstract Objective To establish an HPLC (High Performance Liquid Chromatography) method for simultaneous quantification of aconi-tine, mesaeonitine and hypaconitine in Sini tang. Methods HPLC was performed on a C18 column (4.6 mm×150 mm,5 ×m) with methanol-0.1% triethylamine (65:35) as mobile phase. The flow rate was 1.0 mL·'min-1,column temperature was fixed at 30 ℃ ,and the wave length of detection was set at 240 run. Results For aconra'ne,mesaconitine and hypaconitine,the linear ranges were 0.120 4-6.02 μg (r =0.999 7),0.0412-2.06 μg (r =0.999 5) and 0.080 8-4.04 μg (r =0.999 9),and their average recovery rates were 100.74% (RSD=1.88%),99.9% (RSD=2.98%) and 102.9% (RSD=1.32%),respectively. Conclusion The method could be used for the quality control of Sini Tang,which was available with a good reproducibility.
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