A型激酶锚定蛋白γ在小鼠卵母细胞减数分裂过程中的表达

摘要

Objective In the mammalian oocytes,protein kinase A (PKA) has critical functions in the maintenance of meiotic arrest and oocyte maturation. Spatial regulation of PKA is accomplished by its sequestration via A-kinase anchor proteins (AKAPs). In this study,we aimed to examine the endogenous expression of AKAP7γ in the germinal vesicle (CV) and MII stage of mouse oocytes. Methods The CV and MII stage of mouse oocytes for the presence and modification of the AKAP7γwere examined by Western blot. Results AKAP7γ exhibited a retarded electrophoreoc motility at MII of meiosis compared with CV stage in mouse oocytes. The motility shift of AKAP7γ was susceptible to okadaic acid and alkaline phosphatase. Conclusion Mouse oocytes at MII of meiosis express a phosphorylated form of AKAP7γ. AKAPγ might function in mouse oocytes not only as an anchoring protein but also as a substrate and effector for the anchored kinases.%目的 研究小鼠卵母细胞减数分裂过程中A型激酶锚定蛋白(AKAP7)γ的表达情况.方法 收集生发泡(GV)期及第2次减数分裂中期(MⅡ期)小鼠卵母细胞,Western blot方法 检测AKAP7γ蛋白的表达及修饰情况.结果 在小鼠卵母细胞减数分裂过程中,AKAP7γ在GV及MⅡ期均有表达,且在MⅡ期卵母细胞中,AKAP7γ电泳迁移率变慢,应用冈田酸及碱性磷酸酶分别处理GV及MⅡ期卵母细胞,AKAP7γ电泳迁移率差异消失.结论 随减数分裂进程,在MⅡ期小鼠卵母细胞中,AKAP7γ发生磷酸化修饰,提示AKAP7γ不仅作为AKAP发挥支架蛋白的作用,其本身也可作为其他激酶的底物和效应分子.