Lewis y抗原及胰岛素样生长因子受体1在卵巢浆液性肿瘤中的表达及临床意义

摘要

Objective To study the expression and the clinical significances between Lewis y antigen and insulin-like growth factor receptor 1 (IGF-1R) in ovarian serous tumors. Methods The SP method was used to investigate the positive expression rates of Lewis y and ICF-1R in paraffin-embedded samples of malignant ovarian serous tumors (malignant group), borderline ovarian serous eystadenoma (borderline group),benign serous ovarian cystadenoma (benign group) and normal ovarian tissues (normal group). Double labeling immunofluores-cence method was used to detect the expression of Lewis y and ICF-1R and their correlation was analyzed. Results The positive rates of Lewis y antigen and IGF-1R in malignant tumors were evidently higher than those in borderline and benign tumors and normal tissues (P< 0.05). The expression intensity of IGF-1R in low differentiated tumors was higher than those in moderately and highly tumors (P< 0.05), but there was no statistical significance of Lewis y antigen expression in all the tumors. Positive correlation and synchronization expression were found between Lewis y antigen and IGF-1R in ovarian serous tumor tissues. Conclusion Lewis y and IGF-1R participate in the procedure of malignant transformation of a benign tumor,and strongly correlate with differentiation of ovarian cancer, which is expected to be the diagnostic marker to aid the early diagnosis of ovarian serous cystadenocarcinoma.%目的 探讨Lewis y抗原及胰岛素样生长因子受体1(IGF-1R)在卵巢浆液性肿瘤中的表达及意义.方法 采用免疫组化SP法检测卵巢浆液囊腺癌(恶性组)、交界性卵巢浆液性囊腺瘤(交界性组)、良性卵巢浆液性囊腺瘤(良性组)和正常卵巢组织(正常组)中Lewis y抗原与IGF-1R的表达.用免疫荧光双标记法检测2者在卵巢癌组织中表达的相关性.结果 恶性组Lewis y抗原和IGF-1R的阳性率明显高于交界性纽、良性组及正常组(P＜0.05)；低分化组IGF-1R的表达水平与中、高分化组相比,差异有统计学意义(P＜0.05),而低、中、高分化3组间Lewis y抗原表达差异无统计学意义(P＜0.05).Lewis y和IGF-1R在恶性组中的表达水平增高,具有正相关性(P＜0.05),且2者存在共定位关系.结论 Lewis y抗原及IGF-1R参与了卵巢浆液性肿瘤由良性向恶性的转化与发展,与患者的预后密切相关.