髓样分化因子88抑制剂ST2825对重组耻垢分枝杆菌感染THP-1细胞自噬的影响

摘要

目的：探讨髓样分化因子88抑制剂ST2825对重组耻垢分枝杆菌感染THP⁃1细胞自噬的影响。方法使用髓样分化因子88抑制剂ST2825作用于重组耻垢分枝杆菌感染的THP⁃1细胞，确定ST2825干预的实验组、无ST2825作用的对照组以及空白组。运用荧光显微镜观察自噬小体，RT⁃PCR检测Beclin⁃1基因和Bcl⁃2基因的mRNA的表达。结果与对照组比较，实验组的自噬荧光小点数目明显减少，差异具有统计学意义（P<0.05）；RT⁃PCR检测结果显示Beclin⁃1和Bcl⁃2 mRNA表达较对照组下调。结论髓样分化因子88抑制剂ST2858可能通过干扰Beclin⁃1和Bcl⁃2的分离，抑制THP⁃1细胞自噬。%Objective To investigate the effect of myeloid differentiation factor 88 inhibitor ST2825 on the autophagy of THP⁃1 cells infected by re⁃combinant mycobacterium smegmatis. Methods The myeloid differentiation factor 88 inhibitor ST2825 was applied on the THP⁃1 cells infected by recombinant mycobacterium smegmatis,and three groups were defined:the test group with ST2825 treatment,the control group without ST2825 treatment,and the blank group. Autophagosomes were observed under the fluorescence microscope,and the mRNA expression of Beclin⁃1 gene and Bcl⁃2gene was analyzed by RT⁃PCR. Results Compared with the control group,the number of autophagy fluorescent dots in the test group was ob⁃viously reduced(P<0. 05),and the expression levels of Beclin 1 gene and Bcl⁃2 gene were declined as indicated by the RT⁃PCR detection. Con⁃clusion The myeloid differentiation factor 88 inhibitor ST2825 might inhibit the autophagy of THP⁃1 cells through interfering the separation of Be⁃clin⁃1 and Bcl⁃2.