三种情绪稳定剂抑制哇巴因上调星形胶质细胞ERK1/2磷酸化水平

摘要

目的：探讨锂盐等3种情绪稳定剂对哇巴因上调星形胶质细胞ERK1/2磷酸化水平的作用。方法将培养星形胶质细胞随机分为生理盐水对照组、哇巴因组、情绪稳定剂组（碳酸锂、卡马西平和丙戊酸钠）和哇巴因+情绪稳定剂组。采用Western印迹法半定量检测各组ERK1/2磷酸化水平。结果哇巴因组ERK1/2磷酸化水平升高，与对照组和情绪稳定剂组相比，差异有统计学意义（P<0.05），而哇巴因+情绪稳定剂组与对照组和抑制剂组相比，差异无统计学意义。结论3种情绪稳定剂能抑制哇巴因上调星形胶质细胞ERK1/2磷酸化水平。%Objective To investigate the effets of three mood stabilizers on ouabain⁃induced ERK1/2 phosphorylation in astrocytes. Methods As⁃trocytes were treated with different agents and divided into different groups accordingly,namely,the control group with saline,the group with oua⁃bain,the group with mood stabilizers(lithium carbonate,carbamazepine,sodium valproate)and the group with ouabain+mood stabilizers. The phosphorylation of ERK1/2 in each group was analyzed by Western blot. Results Compared with saline and mood stabilizer groups,the phosphoryla⁃tion of ERK1/2 was increased in the ouabain group,with statistical significance(P<0.05). There was no significant difference in ERK1/2 phosphoryla⁃tion between the group with ouabain+mood stabilizers and the control or mood stabilizer group. Conclusion The three kinds of mood stabilizers can inhibit ouabain⁃induced ERK1/2 phosphorylation in astrocytes.