电针对抑郁模型大鼠五羟色胺及脑源性神经营养因子的影响

摘要

Objective:To investigate the relationship of electroacupuncture(EA)enhance the capability of neural plasticity in depression rats by increasing the expression of brain-derived neurotrophic factor (BDNF) and 5-hydroxytryptamine (5-HT).Methods: Adopt chronic stress depression model rats and stimulate them on Baihui, Yintang with EA.Rats were divided into four groups: control group, model group, EA group and fluoxetine treatment group.Compared with fluoxetine treatment group,Elisa and Western-blot methods were used to investigate the influence of EA on 5-HT levels and BDNF genetic expression.Results:EA can increase the 5-HT level in hippocampal and enhance its BDNF protein expression at the same time.Conclusion:The enhancement of the protein expression of BDNF and 5-HT maybe the anti-depression mechanism of EA.%目的:观察电针对抑郁模型大鼠5-羟色胺(5-HT)、脑源性神经营养因子(BDNF)表达水平的影响,探讨电针改善大脑功能可能存在的机制.方法:采用慢性应激抑郁模型,将大鼠分为空白对照组、抑郁模型组、阳性西药组、电针治疗组(以电针刺激百会、印堂穴)；Elisa法检测各组大鼠海马内5-HT含量、Western-blot法检测海马内BDNF蛋白表达水平.结果:给予慢性应激刺激后,模型组大鼠海马5-HT含量较正常组显著减少(P＜0.01),电针组大鼠海马5-HT含量较模型组显著增加(P＜0.01).Western-blot印迹结果显示与正常组相比,模型组BDNF蛋白表达量显著降低(P＜0.01)；电针组BDNF蛋白表达量较模型组显著增加(P＜0.01).结论:提高海马内5-HT含量及BDNF表达,增强神经可塑性是电针抗抑郁可能的机制.