苁蓉菟丝子煎剂对长期大强度运动大鼠卵巢形态结构的影响

摘要

目的：观察中药苁蓉菟丝子煎剂对长期大强度运动大鼠卵巢组织形态和细胞超微结构的影响，探讨该方剂对运动性月经失调的预防效果及作用机制。方法：将36只3月龄健康雌性SD大鼠随机分为三组：安静对照组（Q 组）、运动对照组（S 组）和中药干预组（M 组），每组12只。S组和 M 组大鼠每日进行一次大强度游泳运动，持续5周，M 组同期每日灌喂中药苁蓉菟丝子煎剂。训练5周后取大鼠卵巢组织，利用 HE染色、透射电镜等技术观察卵巢组织形态和卵巢细胞超微结构。结果：与 Q组相比，S组大鼠卵巢的生长卵泡、成熟卵泡和新鲜黄体数量都显著性减少（ P ＜0.05／ P ＜0.01）而闭锁卵泡增多（P ＜0.05），其颗粒细胞内线粒体、滑面内质网以及高尔基体等细胞器明显减少并含有大量脂滴；M 组大鼠卵巢的生长卵泡、成熟卵泡和新鲜黄体数量则比 S组增加（P ＜0.05／ P ＜0.01）而闭锁卵泡减少（P ＜0.05），其颗粒细胞内线粒体、滑面内质网以及高尔基体等细胞器明显增加而脂滴减少。结论：中药苁蓉菟丝子煎剂可有效预防和改善长期大强度运动对卵巢卵泡发育、成熟以及排卵的抑制作用，改善卵巢颗粒细胞的超微结构，促进其内分泌活动。%Objective:To observe the effect of cistanche and dodder decoction of traditional Chinese medicine on the tissue morphology and cell ultrastructure of ovaries of rats that are engaged in long - term high intensity exercise so as to explore the protective effect of the prescription on athletic menstrual disorder and mechanism of action. Methods:36(3 - month - old)female Sprague - Dawley (SD)rats are divided randomly into three groups:qui-et control group(Q),sport model group(S)and medicine intervention group(M)with12 rats in each group. The rats in the group S and M perform long - term high intensity swimming and the rats in the group M fed with cistanche and dodder decoction daily. Ovarian samples of those rats are obtained after sports training for 5 weeks. Then the techniques of HE straining and transmission electron are used to observe the changes of tissue morphology and cell ultrastructure of ovaries. Results:Compared with group Q,less growing follicles,mature follicles and fresh luteum corpus (P < 0. 05;P < 0. 01)and more atresia follicle (P < 0. 05)can be observed in the group S,but more growing follicles,more mature follicles,more fresh luteum corpus and less atresia follicle are witnesses in the ovaries of group M than those of group S (P < 0. 05;P < 0. 01). Under Transmission Elec-tron Microscope,abundant organelles are found such as mitochondria and endoplasmic reticulum and many lipid droplets in granulose cell in the group Q and M,but in group M,less organelle and more lipid droplets can be observed. Conclusion:The cistanche and dodder decoction can effectively prevent the inhibition of development and maturation of ovarian follicle due to long - term high intensity exercise,improve the ultrastructures of ovari-an cells and promote the secretion activity.