大鼠高架十字迷宫中的行为表现及海马GluR1蛋白水平的性别差异

摘要

目的:探讨雌雄性SD大鼠在高架十字迷宫中各臂的探索时间及其海马内的GluR1蛋白水平是否存在性别差异.方法:将11只雄性和10只雌性SD大鼠放在高架十字迷宫中测试5 min,30 min后快速取出测试大鼠的两侧海马,在-80℃冰箱保存,然后提取蛋白质,用Western印迹方法检测海马内GluR1的蛋白水平.结果:与雄性大鼠相比,雌性大鼠在高架十字迷宫中表现出较轻的焦虑(P＜0.05).雌性大鼠海马总的GluR1水平及左侧海马GluR1水平比雄性大鼠低(P＜0.05).结论:SD大鼠在高架十字迷宫中的行为学表现以及海马内GluR1蛋白水平存在性别差异.%Objective To explore whether sex difference exists in the performance in each arm of elevated plus maze (EPM) and GluRl level in the hippocampus of female and male Sprague-Dawley rats. Methods Eleven male and 10 female SD rats were tested for 5 minutes in the EPM. These rats were decapitated 30 min after testing. The left and right hippocampus were dissected. Samples were stored at - 80 ℃ for protein extracting. Western blot was used to detect the GluRl levels in the hippocampus. Results Female rats exhibited less anxiety-like behaviors than male rats in the EPM (P < 0. 05 ). Female rats had lower GluRl levels in total and left hippocampus than those of male rats (P<0. 05). Conclusion Sex difference exists between female and male rats in the EPM and hippocampal GluRl.