肝素联合肝干细胞经脾移植治疗SD大鼠急性肝损伤

摘要

目的:探讨肝素联合肝干细胞(WB-F344细胞)经脾移植对大鼠急性肝损伤的治疗作用.方法:构建携带绿色荧光蛋白(GFP)基因的慢病毒载体,体外培养、扩增WB-F344细胞,携带GFP基因的慢病毒转染WB-F344细胞.通过腹腔注射四氯化碳(CCl4)建立大鼠急性肝损伤模型,造模后24 h分别将1 mL含2×107个肝干细胞和8 μL肝素的悬液经脾注入大鼠体内.移植术后1 d获取肝脏和脾脏标本进行荧光检测,分别于术后3,7,14和28 d尾静脉抽血送肝功能检测,获取肝脏组织行病理学检测.结果:经慢病毒转染3 d后,WB-F344细胞表达GFP;GFP阳性细胞经脾移植1 d后到达肝脏;实验组大鼠的肝功能和肝脏损伤修复明显优于对照组(P＜0.05).结论:经脾联合肝素和WB-F344细胞移植对CCl4所导致的大鼠急性肝损伤有明显的治疗作用.%Objective To determine the treatment effects of transplanted hepatic progenitor cells (WB-F344 cells) combined with heparin on the acute liver injury in SD rats.Methods A total of 2 × 107 hepatic stem cells (WB-F344) infected with GFP lentivirus and 8 μL heparin were transplanted through the spleen in SD rats with acute liver injury, which was induced by an intraperitoneal injection of CCl4.The liver and spleen tissues underwent fluorescence examination 1 day after the transplantation.The liver functions were tested, and the liver tissues were histopathologically examined on the 3rd, 7th, 14th, and 28th day of the cell transplantation.Results The transfected WB-F344 cells expressed GFP 3 days after the lentivirus infection and were found in the rat liver 1 day after the WB-F344 transplantation.The liver function and histopathological recovery of the liver tissues in the group of WB-F344 transplantation were better than those of the control group (P ＜ 0.05 ).Conclusion Transplantation of hepatic stem cells combined with heparin can promote the liver recovery in rats with acute liver injury induced by CCl4.