直链烷基苯磺酸钠对黑腹果蝇生殖能力及寿命的影响

摘要

Objective To investigate the effect of linear alkylbenzenesulfonate (LAS) on the reproductive capacity and life-span of drosophila melanogaster. Methods Drosophila melanogaster imagoes within 8 h after eclosion were collected with ether anesthesia. The female and male of similar size and normal shape and behavior were selected. The drosophila melanogasters were cultured in the culture medium containing LAS of different densities. We divided the drosophila melanogaster into 4 groups according to LAS concentrations: a low dose group with LAS 150 mg/kg, a middle dose group with LAS 300 mg/kg, a high dose group with LAS 600 mg/kg, and a control group without LAS, respectively. The changes of the reproductive capacity, median lethal time, mean lifespan and max mean life-span of drosophila melanogaster with different doses of LAS were measured and compared with those of the control. Results The pupa numbers of filial generation of drosophila melanogaster in the low, middle,and high dose groups (85.07％ ,84.59％ and 71.88％, respectively) were lower than those in the control group (P ＜0.01 ). The median lethal time, mean lifespan and max mean life-span of drosophila melanogaster in the low, middle, and high dose groups were shorter than those in the control group ( P ＜ 0.05 ). The change of life-span of drosophila melanogaster in the high dose group was remarkable: the median lethal time of female and male shortened 13 days and 15 days, the mean life-span of female and male shortened 18 days and 14 days,and the max mean life-span of female and male shortened 14 days and 12 days, respectively. Conclusion LAS has definite toxicity to drosophila melanogaste, which can degrade the reproductive capacity of drosophila melanogaste and shorten the life-span of drosophila melanogaste.%目的:探讨直链烷基苯磺酸钠(linear alkylbenzenesulfonate,LAS)对果蝇生殖能力及寿命的影响.方法:采用乙醚麻醉的方法,收集8 h内羽化而未交配的野生型黑腹果蝇,选择个体大小相近及外观行为正常的果蝇进入实验,雌雄各半.在培养基中加入LAS并按不同浓度分组(低剂量组:150 mg/kg,中剂量组:300 mg/kg,高剂量组:600 mg/kg,对照组:不添加LAS).测定培养基中含有不同浓度LAS时果蝇繁殖能力及半数死亡天数、平均寿命及平均最高寿命,并与对照组进行比较.结果:低剂量组、中剂量组、高剂量组果蝇的子代数量分别为对照组的85.07%,84.59%和71.88%,与对照组相比,差异有统计学意义(P<0.01);各剂量组雌、雄果蝇的半数死亡天数、平均寿命及平均最高寿命与对照组相比均有不同程度缩短,高剂量组所受影响最为显著,雌、雄性果蝇的半数死亡时间分别缩短13与15 d,平均寿命分别缩短18和14 d,平均最高寿命分别缩短14和12 d.结论:LAS对果蝇有一定毒性,可引起果蝇生殖能力降低及寿命缩短.