温度和光照强度对银杏叶黄酮合成的影响

摘要

In order to reveal the influence of temperature and light intensity onflavonoid biosynthesis in ginkgo leaves, two-year old ginkgo potted seedlings was treated under three levels of temperature and light intensities. The contents offlavonoid and key enzyme gene expression were measured then the correlation analysis offlavonoid contents and key enzyme gene expression was built. The result showed thatflavonoid contents per plant were significantly increased at T1Q3 for 40d and T2Q2 for 20 d;In most treatments, the increment offlavonoid showed significant temperature effect, while just in few treatments, the increment offlavonoid showed significant light intensity and temperature´light intensity effect; the key enzyme gene expression contents showed significantly temperature, light intensity and temperature´ light intensity effect; there is remarkable positive correlation between quercetin contents and expression of F3H,FLS andANS after 10 d treatment.%为了探索温度和光照强度对银杏叶黄酮合成及调控机制，本研究对两年生银杏盆栽苗进行温度和光强的两因素三水平的处理，测定黄酮类黄酮类化合物的含量、代谢过程中关键酶基因表达量并建立黄酮类含量与基因表达的相关性，结果表明：T1Q3处理40 d后和T2Q2处理20 d后，单株银杏黄酮含量相较处理前显著增加；大部分处理中，黄酮类化合物的增量存在显著的温度效应，部分处处理中，黄酮类化合物的增量存在显著光强效应和温度、光强交互效应；黄酮代谢过程基因表达量存在显著或极显著的温度、光强及温度、光强交互效应；相关性分析表明；仅在处理10 d时，槲皮素的含量与F3H、FLS和ANS基因的表达量呈现明显的正相关。