Snail与Claudin-3蛋白的表达与原发性肝癌发生及预后的关联性研究

摘要

Objective This study aimed to investigate the relationship between the expression of Claudin-3 or Snail and the clinical pathology and prognosis in the hepatocellular carcinoma (HCC) patients by detecting the expression of Claudin-3 and Snail in the HCC and liver cancer-adjacent tissues. Methods Snail and Claudin-3 were detected in cases of 60 liver cancer-adjacent tissues and 82 cases of HCC by using immunohistochemical technique. The postoperative prognostic factors were assessed by univariate Kaplan-Meier analysis and a multivariate Cox proportional hazard model. Results S nail positive expression was 69.47％ in HCC and was significantly higher than liver cancer-adjacent tissues (P = 0.001). Claudin-3 proteins positive expression were 47.61％ in HCC and were significantly lower than liver cancer-adjacent tissue (P = 0.002). The expression of Snail and Claudin-3 was negatively correlated (r =-0.430,P＜ 0.01). Snail positive expression in HCC was significantly higher than liver cancer-adjacent tissues, and the difference was statistically significant in microvascular invasion and stages of pathology tumor lymph node metastasis (P＜ 0.05). Multivariate analysis by using Cox regression model identified that Snail and Claudin-3 were independent prognostic factors of HCC (P＜ 0.05). Conclusion The correlation between the Snail or Claudin-3 and the occurrence and invasion in HCC were exhibited. The combined detection of Snail and Claudin-3 protein in HCC patients might be an important pathological and prognosis biomarker in HCC patients.%目的 本研究通过收集原发性肝细胞癌(hepatocellular carcinoma, HCC)患者的临床资料,并检测癌及癌旁组织中Claudin-3和Snail蛋白的表达,探讨Claudin-3和Snail蛋白与肝癌患者临床病理特征与预后的关联.方法 收集根治性切除的肝细胞癌病例患者资料,利用免疫组织化学的方法检测82例HCC组织及60例癌旁组织中Snail、Claudin-3蛋白的表达.采用Kaplan-Meier法进行生存分析,应用Cox比例风险模型进行多因素回归分析.结果 肝癌组织中Snail蛋白阳性表达69.47％,高于癌旁组织,差异有统计学意义(P=0.001);Claudin-3阳性表达47.61％,低于癌旁组织,差异有统计学意义(P=0.002);两者的表达呈负相关-0.430,P＜0.01);Snail蛋白在HCC中阳性表达明显高于癌旁组织,在病理分期、微血管侵犯相关方面差异有统计学意义(P＜0.05);在Cox多因素回归分析中,Snail、Claudin-3的表达是HCC患者生存的独立危险因素(P＜0.05).结论 Snail与Claudin-3蛋白与HCC的发生、侵袭有一定的关联性.进行HCC患者Snail、Claudin-3蛋白的联合检测,在临床实践中对HCC患者病理学特征与预后的判断具有重要的提示作用.