目的 通过对骨标本不同固定和脱钙方式的比较,探索适合于骨标本的固定和脱钙方式.方法 分别采取先固定后脱钙、同时固定加脱钙和先脱钙后固定这三种方式对50例长骨肿瘤标本进行对比分析.结果 先固定后脱钙组常规HE染色效果优于同步固定脱钙组,但两组比较差异无统计学意义(P>0.05),该两组常规染色效果均明显优于先脱钙后固定组(P<0.01).结论 对骨组织常规HE制片来说,骨组织固定的方式很重要,脱钙应该先固定后脱钙或脱钙固定同时进行,建议不采用先脱钙后固定的方式处理骨组织.%Objective; Through comparing different fixation and decalcification methods for bone specimens, to explore the a-daptive fixation and decalcification method for bone tissue. Methods; Three different ways including decalcification after fixation (DAF) , decalcification while fixation(DWF) , decalcification before fixation( DBF) were conducted on 50 cases of bone specimens and the results were compared. Results: The routine HE staining results of the DAF group was better than that of the DWF group while the difference was not statistically significant(P > 0. 05) . The routine HE staining results of the DAF and DWF group were better than that of the DBF group with significantly difference(P <0. 01) . Conclusion; For the routine HE slide of bone tissue, the way of fixation is very important. Decalcification should be conducted before fixation or performed simultaneously with fixation. The DBF methodis not recommended.
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