EAE大鼠胸腺CD4+CD25+Foxp3+Treg细胞的动态变化及α-硫辛酸的干预作用

摘要

目的 探讨实验性变态反应性脑脊髓炎(EAE)大鼠不同病程中胸腺CD4+ CD25+ Foxp3+Treg细胞变化情况及α-硫辛酸对EAE大鼠胸腺的干预作用.方法 取不同时期对照组、自然病程EAE组及α-硫辛酸EAE组大鼠的胸腺组织做流式细胞学,动态检测CD4+ CD25+ Foxp3+Treg细胞的变化情况.结果 EAE组大鼠急性期、复发期CD4+ CD25+ Foxp3+Treg细胞较同时期对照组明显减少(P<0.05),缓解期有所上升;α-硫辛酸组与同期EAE组相比CD4+ CD25+ Foxp3+Treg细胞无明显变化;半年期三组大鼠胸腺CD4+ CD25+Foxp3+Treg细胞都明显下降,各组间无统计学差异.结论 CD4+CD25+ Foxp3+Treg细胞参与了EAE的发病,与病程的发展密切相关;α-硫辛酸对EAE大鼠的干预作用并非通过CD4+CD25+ Foxp3+Treg细胞发挥其治疗作用;随着年龄的增长,胸腺不再是机体的主要免疫器官.%Objective To observe the variation of regulatory CD4 + CD25 + Foxp3 + T cell( Treg ) of thymus in different courses of experimental autoimmune encephalomyelitis ( EAE ) group, alpha lipoic acid ( ALA ) group and investigate the immune effect of ALA on the thymus of EAE. Methods Flow cytometry was used to analyze the expression rate of CD4 + CD25 + Foxp3 + Treg cells in the course of control group, EAE group and ALA group. Results In acute course and relapse course of EAE group, the expression of CD4 + CD25 + Foxp3 + Treg cells decreased significantly than that in the control group ( P <0. 05 ). In recovery course CD4 + CD25 + Foxp3 + Treg cells raised. No significant difference was found in the expression rate of CD4 + CD25 + Foxp3 + Treg cells between the EAE group and the ALA group. The three groups in half-year course , CD4+ CD25 + Foxp3 + Treg cells reduced to a low level significantly ,but there was no significant difference among the three groups( P > 0. 05 ). Conclusion CD4 + CD25 + Foxp3 + Treg cells may play a role in the occurrence of EAE. There is significant relation between the development of EAE and CD4 + CD25 + Foxp3 + Treg cells. The function of ALA may not play through CD4 + CD25 + Foxp3 +Treg cells in immune adjustment at EAE. As the age added,the thymus may not be the main immune organ.