目的 应用Fluoro-Jade C(FJC)染色方法 在小鼠匹罗卡品癫痫模型中检测梨状皮质结构中神经元的变性情况,以了解梨状皮质结构在慢性颞叶癫痫发生中的病理变化和癫痫反复发作的神经基础.方法 雄性昆明小鼠10只(对照组5只,匹罗卡品处理组5只).处理组在癫痫持续状态后3d处死处理组小鼠.在梨状皮质水平切制冠状切片,行FJC染色,在荧光显微镜下观察FJC阳性细胞的形态和在梨状皮质中的整体分布情况.结果 在处理组,FJC染色的脑切片上可以很清楚地看到呈亮黄绿色荧光的FJC阳性细胞,呈神经元形态,胞体和突起均清晰显示.在梨状皮质和梨状内核内出现大量FJC阳性细胞,而对照组未见.结论 在小鼠匹罗卡品癫痫模型中运用FJC染色技术显示梨状皮质内发生了大量神经元变性,此研究有利于更好地理解颞叶癫痫中中枢神经系统所发生的长期病理变化和自发反复发作的癫痫机制.%Objective To detect degenerative neurons by Fluoro-Jade C ( FJC ) that my' occur in the piriform cortex of pilocarpine-treated mice and to understand the neural basis of piriform cortex in long-term brain pathological changes and recurrent seizure mechanism of chronic epilepsy. Methods Male adult mice ( Kunming stain, n = 10 ) were divided into pilocarpine-treated group ( n = 5 ) and controls ( n = 5 ). Pilocarpine-treated mice were sacrificed at 3 d after status epilepticus. Brain coronal sections in the piriform cortex levels were subject to FJC staining. Finally, FJC-stained brain sections were examined under an epifluorescence microscope to identify the shape and overall distribution of the FJC positive cells in the piriform cortex. Results The FJC-positive staining cells showed a bright green color in the somas and fine processes with neuronal profiles. Numerous degenerating cells could be seen in the piriform cortex including the endopirifonn nucleus. They were not detected in control animals. Conclusion This study successfully apply FJC staining to demonstrate massive neurodegeneration in the piriform cortex, which will benefit further understanding of long-term brain pathological changes and recurrent seizure mechanism in temporal lobe epilepsy.
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