钙信号早期干预对大鼠心肌细胞钙调素依赖性蛋白激酶信号途径及细胞凋亡的影响

摘要

通过建立肥大心肌细胞模型，研究钙通道阻滞剂对心肌细胞钙调素依赖性蛋白激酶II （calcium/calmodulin-dependent protein kinase II，CaMKII）的表达及钙离子浓度的变化对钙泵、ATP凋亡信号调节激酶（apoptosis signal-regulation kinase1 ASK1）、细胞活力及细胞凋亡率的影响，深入了解钙离子拮抗剂在心肌细胞肥大过程中的作用。通过早期应用钙离子拮抗剂，可以明显改善CaMKII信号系统的活性，保持心肌细胞内钙稳态，抑制心肌细胞的凋亡。%To establish the animal′s models of hypertrophied cardiomypcytes,to investigate the effect of L-type calcium channel blockers on the expression of calmodulin-dependent protein kinase II(CaMKII),the Ca2+-ATPase,ATP,ASK1,cell viability and apoptotic in myocardial cells.,in order to understand calcium antagonists in the process of myocardial hypertrophy role thoroughly.It can significantly improve CaMKII signal system activity,and keep the myocardial intracellular calcium homeostasis,inhibiting myocardial apoptosis by the early application of calcium antagonists.