霍夫变换在 PCR -反向点杂交检测结果自动化判读中的应用

摘要

To explore the application of Hough transform for automated interpretation of the results of polymerase chain reaction -reverse dot blotting (PCR -RDB)for the detection of genetic mutations.The Hybribio HMM-2I Rapid Hybridizer for the detection of DNA mutations was used as an example.A method was established for fast interpretation of the PCR -RDB results by image recognition using Hough transform.In addition,a set of mobile -terminals -based results processing scheme were proposed.A software was de-veloped for automatic interpretation of the PCR -RDB results for detecting common mutations in 113 thalassemia cases and subtypes of human papilloma virus in 83 cases.The results were compared with those of manual interpretation,with a consistency of 100%.The Hough transform can suit automated interpretation of the results of PCR -RDB assays.The method does not require human interven-tion,can reduce the error rate of visual interpretation,and improve the efficiency of testing.%探讨霍夫变换（Hough transform）在聚合酶链反应-反向点杂交（polymerase chain reaction - re-verse dot blotting，PCR -RDB）检测基因突变结果的自动化判读中的应用。以凯普 HMM-2I 医用核酸分子快速杂交仪检测基因突变为例，建立一种通过霍夫变换进行图像识别的方法，以快速判读 PCR -RDB 的检测结果，并提出一套基于移动终端的检测结果后处理方案。利用开发的软件，对113份地中海贫血常见突变以及86份人乳头瘤病毒分型的 PCR -RDB 检测结果进行了自动读取，并同人工判读结果进行了比较，二者的符合率均达100％。将霍夫变换应用于 PCR -RDB 检测图像的自动化判读符合实际需求，无需人工干预，可以降低肉眼判读的错误率并提高工作效率。