用超滤法从猪血中分离纯化猪血红蛋白

摘要

In order to obtain a high purity and concentration of porcine hemoglobin from the fresh swine blood to support the artificial red blood cell research, method with ultrafiltration separating was applied. The purified hemoglobin was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis ( SDS-PAGE) and ultra-visable spectrophotometer. The ultrafiltration condition was under operating pressure 0. 05Mpa at 12℃ . And the results indicated that the purity of the porcine hemoglobin separated by ultrafiltration was higher than 99％ and the concentration of the hemoglobin was 20 times higher than that before purification.%为了给人工红细胞的研究提供较高纯度和浓度的血红蛋白,采用超滤膜分离的方法,通过研究分析,在一定超滤条件,从新鲜猪血中分离纯化得到猪血红蛋白.并应用SDS-PAGE凝胶电泳、紫外-可见分光光度计对纯化后的血红蛋白进行了鉴定分析.经分析,优化的超滤条件为:操作压力0.05Mpa、料液温度12℃;在此条件下分离纯化的猪血红蛋白.纯度达到了99%以上.浓度较纯化前提高了近加倍.