四尾栅藻对铜绿微囊藻光合活性及毒素产生与释放的影响

摘要

Mixed culture of Microcystis aeruginosa and Scenedesmus quadricanda was carried out to research the influence of Scenedesmus quadricanda exerting upon Microcystis aeruginosa on toxins producing and photosynthetic capacity.The control group was the culture of Microcystis aeruginosa only.Counter Star was used to obtain the biomass of the algaes.The maximum chlorophyll fluorescence quantum yield(Fv/Fm) was measured by Water-PAM after dark adaptation.The intracellular microcystin-LR(MC-LR) of Microcystis aeruginosa was extracted by boiling water bath at 100℃ for 20 minutes.The extracellular and intracellular MC-LR content was detected with high performance liquid chromatographic (HPLC).Result showed that during the whole growth stage the biomass of Microcystis aeruginosa was higer in the control group.But both of the extracellular and intracellular MC-LR contents were lower in the control group than that in the experimental group.The value of the yield of mixed cultured algae was higher than that of the pure cultured Microcystis aeruginosa.The results proved that the existence of Scenedesmus quadricanda influenced the Microcystis aeruginosa on biomass,photosynthetic activity,toxins producing and releasing.Each Microcystis aeruginosa cell would produce and release much more toxins when mixed and cultured with Scenedesmus quadricanda.When the value of yield rised,the biomass of the algae cells increased logarithmically and the value of yield reached the maximum.%采用将铜绿微囊藻与四尾栅藻混合培养的方法,初步研究了四尾栅藻对铜绿微囊藻生长、产毒及光合作用能力的影响.对照组为铜绿微囊藻单种培养.以细胞计数仪测藻生物量,Water-PAM测定暗适应后最大叶绿素荧光量子产量Yield(Fv/Fm),高效液相色谱分析细胞内及细胞外微囊藻毒素MC-LR浓度.结果表明:在生物量上,整个生长过程中铜绿微囊藻单种培养比与四尾栅藻混合培养大,而胞外及胞内毒素MC-LR浓度始终是前者低于后者.混合藻的Yield值总是大于单种培养的铜绿微囊藻.实验结果证明四尾栅藻的存在对铜绿微囊藻生物量、光合作用活性、毒素产生与释放均有影响.混合培养时,最大生物量提前,且数值比单种培养低,每个细胞能产生并且释放更多的毒素.Yield值上升时,生物量呈对数增长,Yield达到最大则生物量数量级达到最大,不再增加.