二倍体马铃薯叶肉细胞原生质体培养的研究

摘要

以马铃薯抗青枯病二倍体材料ED13和CE171、炸片颜色好的二倍体材料HS66以及优良性状双单体材料DH401和DH405为供体材料，对马铃薯叶肉原生质体培养进行研究。叶片悬浮黑暗预处理和试管苗黑暗预处理两种预处理方式对原生质体活力无显著影响。以0.5 mol/L甘露醇为渗透调节剂，25℃酶解12 h条件下，适宜CE171和DH401纤维酶浓度略高于ED13、HS66和DH405，分别为0.3％和0.4％。 ED13和DH401原生质体在VKM液体培养基中培养3～4周，经愈伤组织生长培养基培养2周，转至芽诱导培养基培养，2～3个月后形成具根茎叶的完整植株。 HS66和CE171原生质体培养6～8周也能形3～4 mm愈伤组织，但没有分化出芽；DH405的原生质体不分裂。%The studies on protoplast culture for diploid potato with resistant to bacterial wilt or with good chip color ( ED13 , CE171 , and HS66) and ones with good agricultural traits (DH401 and DH405) showed that there were no significant difference of protoplast yield and viability between leaves in suspension medium in dark and complete plantlets in dark for pretreatment .When 0.5 mol/L mannitol was used as an osmosis regulator , the suitable cellulose concentration (0.4%) for protoplast isolation of CE171 and DH401 was higher than those of ED13, HS66 and DH401(0.3%) at 25℃ for 12 h.The protoplast of ED13, DH401, CE171, and HS66 could be induced into callus in VKM liquid medium .The protoplast of ED13 and DH401 could regenerate into complete plantlets , but protoplast of DH405 had no division capability .