高碱度和高氨对达里湖瓦氏雅罗鱼耗氧率和排氨率的影响

摘要

Using hydrostatic water in sealed chamber for measurement , this study compared the change of oxygen consumption rate and ammonia excretion rate of Leuciscus waleckii Dybowski (72.62 ±3.70)g exposed in high carbonate alkalinity water (CA 50 mmol/L) and high ammonia water (NH4CO3 1.5 mmol/L) for 4 time points (2 h, 4 h, 6 h and 8 h) measurement.Oxygen consumption rate was significantly inhibited in carbonate alkalinity water and high ammonia water when compared that in fresh water ( P<0.05 ) .No significant difference of the oxygen consumption rate between carbonate alkalinity and ammonia water treated fish was detected for the last three tested time points, 4, 6, and 8 except for the first testing time point.Results of ammonia excretion rate showed that com-pared with those in fresh water , the ammonia excretion rate of fish exposed in carbonate alkalinity water and ammonia water was signifi -cantly inhibited ( P<0.05 ) .In ammonia water , the ammonia excretion rate of L.waleckii Dybowski for all 4 time points decreased , which had an extremely significant difference compared with fresh water and carbonate alkalinity water .Energy metabolism ( O∶N) study found that there had no significant difference in oxygen-nitrogen ratio in fish between fresh water and carbonate alkalinity water . However , compared with fish in fresh water and carbonate alkalinity water , fish in ammonia water had an extremely significant differ-ence in oxygen-nitrogen ratio .%采用静水密闭的方法，比较了体质量为（72.62±3.70）g达里湖瓦氏雅罗鱼（Leuciscus waleckii Dybowski，简称雅罗鱼）在高碱度（NaHCO350 mmol／L）和高氨（NH4CO31.5 mmol／L）胁迫下处理4个时间点（2 h、4 h、6 h和8 h）耗氧率和排氨率的变化。耗氧率实验结果表明：与淡水组相比，高碱度和高氨显著抑制了雅罗鱼的耗氧率（P＜0.05）；高碱度组与高氨组相比，雅罗鱼耗氧率除了在监测的第1个时间点内存在显著性差异外（P＜0.05），其他时间点无明显差异（P＞0.05）。排氨率实验结果显示：与淡水组相比，高碱度和高氨均显著抑制了雅罗鱼的排氨率（P＜0.05）。其中高氨组排氨率在所监测的4个时间点内全部下降，并与淡水组和高碱度组相比存在极显著差异（ P＜0.01）。能量代谢研究发现，淡水组和高碱度组氧氮比均无显著差异（ P＞0.05）；与淡水对照组和高碱度组比较，高氨组氧氮比差异极显著（ P＜0.01）。