不同刺激剂对健康人和结核患者外周血T细胞产生γ干扰素和肿瘤坏死因子-α的影响

摘要

Objective:To detect the changes of the different stimulants on the peripheral blood T cell subsets of healthy people and tuberculosis( TB) patients producing interferon-γ( IFN-γ) and tumor necrosis factor-α( TNF-α) . Methods:The proportion of T cell subsets producing INF-γ and TNF-α in 12 healthy people peripheral blood mononuclear cells and 12 TB patients peripheral blood stimulated with PMA combined with ionomycin, Mtb-Hag, PHA and blocked with monensin were detected using the flow cytometry. Results:The proportion of INF-γ produced by αβ T cells of peripheral blood stimulated with PMA in healthy people was significantly higher than that in TB patients(P<0. 05). The proportions of INF-γproduced byγδT cells of peripheral blood stimulated with PMA, Mtb-Hag and PHA in healthy people were higher than that in TB patients(P<0. 05 to P<0. 01). The proportions of TNF-αproduced byαβ T cells of peripheral blood stimulated with PMA PHA in healthy people were higher than those in TB patients(P<0. 05). The proportions of INF-α produced byγδT cells of peripheral blood stimulated with PMA and PHA in healthy people were higher than that in TB patients(P<0. 05 ). Conclusions:After stimulated with different stimulants,the proportion of INF-γ produced by γδ T cells in healthy people is higher than that in TB patients. After simulated with PMA and PHA,the proportion of TNF-αproduced byαβT cells and γδ T cells in healthy people is higher than that in TB patients.%目的：：检测健康人和结核患者外周血经不同刺激剂刺激后T细胞亚群产生γ干扰素( IFN-γ)和肿瘤坏死因子-α( TNF-α)的变化。方法：12名健康人和12例结核患者的外周血单个核细胞用佛波醇脂( PMA)+钙离子霉素( IM)、结核杆菌耐热抗原( Mtb-HAg)、植物血凝素( PHA)刺激，莫能霉素阻断，流式细胞仪检测产生IFN-γ和TNF-α的T细胞亚群比例。结果：PMA+IM刺激健康人外周血αβ T细胞产生IFN-γ的比例明显高于结核患者(P<0.01)；PMA+IM、Mtb-HAg和PHA刺激健康人外周血γδ T细胞产生IFN-γ的比例高于结核患者(P<0.05~P<0.01)。 PMA+IM和PHA刺激健康人外周血αβ T细胞产生TNF-α的比例均高于结核患者(P<0.05)；PMA+IM和PHA刺激健康人外周血γδ T细胞产生TNF-α的比例均高于结核患者(P<0.05)。结论：经不同刺激剂刺激后，健康人γδ T细胞产生IFN-γ的比例高于结核患者γδ T细胞产生IFN-γ的比例。经PMA和PHA刺激后，健康人αβ T和γδ T细胞产生TNF-α的比例高于结核患者。