清热解毒药配伍桔梗汤对急性肺损伤模型大鼠TLR4 mRNA表达的影响

摘要

Objective To observe the influence of Chinese herbal remedies (CHR) with the actions of clearing away heat to remove toxin combined with Jugeng Decoction on the expression of lung toll-like receptor 4 (TLR4) mRNA in rat model of acute lung injury (ALI) induced by endotoxin, and to discuss the synergy mechanism of Jugeng Decoction. Method All male SD rats were divided into the normal saline group, ALI model group, CHR group and CHR combined with Jugeng Decoction group according to the table of random number. The rat model of ALI was established with the femoral venous injection of lipopolysaccharide (LPS). The serum contents of tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10), the activity of lung myeloperoxidase (MPO) and the expression of TLR4 mRNA were detected in all groups. Result In the ALI model group the serum contents of TNF-α and IL-10 were higher significantly than those in the normal saline group ( P < 0.01 or P <0.05), and MPO activity and TLR4 mRNA expression were enhanced significantly compared with those in the normal saline group ( P <0.01 or P <0.05). After the intervention of CHR the serum contents of TNF-α and IL-10, MPO activity and TLR4 〖JP3〗mRNA expression decreased significantly ( P <0.01 or P <0.05), and the curative effect of the CHR combined with Jugeng Decoction group was better than that of the CHR group ( P <0.05). Conclusion Jugeng Decoction can improve the effect of CHR with the actions of clearing away heat to remove toxin in the reduction of serum contents of TNF-α and IL-10 and inhibition of MPO activity and TLR4 mRNA expression.%目的 观察清热解毒中药配伍桔梗汤对内毒素致急性肺损伤(ALI)模型大鼠肺Toll样受体4(TLR4) mRNA表达的影响,探讨桔梗汤的配伍增效机制.方法 雄性SD大鼠按随机数字表法分为生理盐水组、ALI模型组、清热解毒药组、清热解毒药加桔梗汤组.股静脉注射内毒素脂多糖制备ALI大鼠模型,检测各组大鼠血清肿瘤坏死因子-α(TNF-α)、白细胞介素-10(IL-10)含量及肺组织髓过氧化物酶(MPO)活性、TLR4 mRNA的表达.结果 与生理盐水组相比,ALI模型大鼠血清TNF-α、IL-10含量明显升高(P<0.01或P<0.05),肺组织MPO活性、TLR4 mRNA表达明显增强(P<0.01或P<0.05).中药干预后大鼠血清TNF-α、IL-10含量及肺组织MPO活性、TLR4 mRNA表达均明显下降(P<0.01或P<0.05),且清热解毒药与桔梗汤配伍疗效优于单纯清热解毒药(P<0.05).结论 桔梗汤对清热解毒药降低血清TNF-α、IL-10含量、抑制MPO活性及TLR4 mRNA基因表达、发挥解毒抗炎功效,具有促进作用.