基于DPPH、FRAP法的薄荷药材抗氧化谱效关系研究∗

摘要

目的：通过研究薄荷药材特征图谱与其抗氧化活性的相关关系,探讨薄荷抗氧化活性物质基础。方法采用1,1-二苯基-2-三硝基苯肼(DPPH)、总抗氧化能力评估法(FRAP)法分别测定薄荷样品抗氧化活性,同时测定样品相应的特征图谱,通过偏最小二乘法研究特征图谱色谱峰和抗氧化活性相关性,筛选活性色谱峰,并结合HPLC-MS/MS n 进行结构鉴定。结果筛选得到的橙皮苷、香叶木苷、蒙花苷、百里香新、黄姜味草酸等6个化合物与清除DPPH自由基能力相关,橙皮苷、蒙花苷、反式丹酚酸J、迷迭香酸等8个化合物与总抗氧化能力相关。结论谱效关系研究能够表征中药化学成分-药效相关关系,可以较为快捷地从复杂体系中筛选活性化合物。%Objective To explore the active ingredients of Menthae Haplocalycis Herba by investigating the correlation between HPLC spectrum and antioxidant activity. Methods We established DPPH[1,1-Diphenyl-2-picrylhydrazyl radical 2,2-Diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl,DPPH] and FRAP (ferric ion reducing antioxidant power, FRAP) assay to determine the antioxidant activity of Menthae Haplocalycis Herba. The spectrum-effect correlation was studied by using PLS(Partial least squares). HPLC-MS/MSn was applied to identify their structures. Results Six compounds including hesperidin, diosmin, buddleoside, thymusin and xanthomicrol, which made great contributions to the DPPH scavenging activity, were screened; eight compounds, including hesperidin, buddleoside, rosmarinic acid and trans Salvianolic acid J, were obtained which made great contributions to the total antioxidant activity. Conclusion It is an effective way to characterize spectrum-efficacy correlation and clarify the active components of Chinese medicinal.