HIV-1整合酶活性检测方法的研究进展

摘要

HIV-1整合酶介导病毒DNA与宿主染色体的整合,是病毒复制周期中必不可少的环节,抑制整合酶能有效阻止病毒在体内复制.同时,人体细胞中无整合酶的功能类似物,因此,整合酶是抗HIV-1的理想靶点.近年来,整合酶活性的体外检测方法得到快速发展,并广泛应用于整合酶活性检测、反应机理研究及整合酶抑制剂的体外筛选等研究中.本文综述了整合酶活性检测方法的研究进展.%HIV-1 integrase catalyzes the integration of virus DNA into the host chromosome, which is a crucial step in viral replication. The inhibition of integrase activity is an effective measure to prevent HIV-1 replication.Moreover, integrase has no known functional analogue in human cells. Therefore, integrase becomes an ideal target for anti-HIV therapy. During the past decade, in vitro assays have been developed and applied to monitor the enzymatic activity of the integrase, study the reaction mechanisms of integase and screen integrase inhibitors in vitro. This review describes the recent development on integrase activity assays.