低温诱发肉鸡腹水综合征过程中缺氧诱导因子-1α的动态表达

摘要

[目的]通过测定环境低温诱发AS发生发展过程中缺氧诱导因子-1α表达的动态变化,初步探讨HIF-1α与AS发生发展之间的关系.[方法]100只商品代肉公雏,15d时随机分为常温对照组(22～24℃)和低温组(9～11℃).各组在22、29、36和43 d随机选取6只鸡,测定mPAP、AHI和心、肺组织中HIF-1α mRNA的表达量,试验结束后统计腹水发生率.[结果]低温组AS发生率(22％)显著高于对照组(4％) (P＜0.05);低温组肉鸡mPAP和AHI显著或极显著高于对照组(P＜0.05,P＜0.01);低温组肉鸡心脏HIF-1αmRNA表达量显著或极显著(P＜0.05,P＜0.01)增加;肺脏HIF-1α mRNA表达量类似于同时间点的心脏.[结论]低温诱发AS发生过程中,肉鸡心脏和肺脏HIF-1α表达量明显增加,可能参与肉鸡AS的发生发展.%[Objective] In order to investigate the relationship between HIF-1α and ascites syndrome,the dynamic expression of HIF-1a mRNA in hearts and lungs of broiler chickens was measured in the study.[Methods] 100 male broiler chickens of commercial strain were randomly allocated to control group (raised at the temperature of (22-24)℃) and low temperature group (raised at the temperature of (9-11)℃) at 15 days old.At 22,29,36 and 43 days of age,six broilers in each group were sampled.Pulmonary arterial pressure (PAP),ascites heart index (AHI) and expression of HIF-1α mRNA in lungs and hearts were determined.And total incidence rates of AS were counted at 15-43 days of age.[Results] The experimental results showed that low ambient temperature induced pulmonary hypertension (higher mPAP),right ventricular hypertrophy (greater AHI) and ascites (22％ incidence rate) in broiler chickens.Meanwhile,the expression levels of HIF-1α mRNA in lungs and hearts were significantly increased (P ＜ 0.05 or P ＜ 0.01) at different time points (22,29,36 and 43 days of age) in low ambient temperature compared with control group.[Conclusions] Our findings hinted that low temperature could increase the expression of HIF-1 alpha mRNA in hearts and lungs of broiler chickens,and HIF-1 alpha might be associated with the phase of development of AS.