两肾一夹型高血压合并2型糖尿病大鼠模型的实验研究

摘要

Objective :Establishing a compound model with renal vascular hypertension combined type 2 diabetic rats so as to observe its stability by monitoring the blood and pressure in different time periods .Methods :First ,we selected Male SD rats and adopted two kidney one clip method and drinking 1 .5% saline to establish hypertensive rat model ,on the basis of this model ,at the 4th week in high glucose ,and high fat feeding ,we injected abdominal cavity with strepto_zotocin (STZ 35mg/kg) .Second ,we continued to feed it with high -sugar high-fat diet until the experiment ended .Fi_nally ,we observed the changes of blood pressure ,fasting blood glucose(FBG) ,fasting insulin (FINS) ,lipids ,serum C- reactive protein (CRP) ,insulin sensitivity index (ISI) in different time points .Meanwhile ,we observed the model e_valuation in general situation .Results :About 70% of renal vascular hypertension combined type 2 diabetic rats were made into models ,all the succeed models had a stable blood pressure and blood glucose from its established to the end of the experiment .It has a statistically significant difference compared with the control group ,sham operation group(P <0 .05) .Conclusion :Successfully replicated hypertension combined with type 2 diabetic rat model ,which has the advantage of high economic value ,easy operation ,high modulus and good stability .%目的：建立肾血管性高血压合并2型糖尿病大鼠复合模型，通过检测不同时段的血压、血糖来观察此模型的稳定性。方法：雄性SD大鼠先采用两肾一夹法（2K1C ）并饮用1．5％盐水建立高血压大鼠模型，在此模型成模的基础上喂养高糖、高脂饮食4周后经腹腔注射链尿佐菌素（35 mg／kg），然后继续予高糖、高脂饮食饲养至实验结束，检测不同时间点血压、空腹血糖、空腹胰岛素、血脂、血清C－反应蛋白，计算胰岛素敏感指数，并观察一般情况对所建模型进行评价。结果：高血压合并糖尿病组大鼠成模率70％，其血压、血糖值达到成模标准，至实验结束时较稳定，分别与假手术组、空白对照组比较差异有统计学意义（ P ＜0．05）。结论：成功复制高血压合并2型糖尿病大鼠模型，该模型经济、操作简单、成模率高、稳定性好。