蒙药童格勒格-1对高脂大鼠的降脂作用及其机制研究

摘要

目的：探讨蒙药童格勒格-1（TGLG -1）对高脂大鼠血脂的调节作用及对大鼠脂肪组织肿瘤坏死因子-α（TNF -α）表达的影响。方法：以高脂饲料喂养大鼠1个月建立高血脂动物模型，用 TGLG -1灌胃，6周后测定大鼠血脂和脏器系数的改变，并观察肝脏的形态变化；用免疫组织化学法检测脂肪组织 TNF -α的表达及脂肪细胞数目的变化。结果：高脂大鼠血清总胆固醇（TC）、甘油三酯（TG）水平高于对照组（ P <0.01），且肝组织出现明显的脂肪变性，脂肪细胞体积增大且数目减少，说明模型制作成功；用蒙药 TGLG -1治疗6周后，大鼠血清 TC、TG 水平降低，高密度脂蛋白胆固醇（HDL - C）水平升高（ P <0.01），肝组织脂肪变性有所缓解；免疫组织化学检测结果显示，高脂大鼠脂肪组织TNF -α表达高于其它3组，而蒙药 TGLG -1组表达最低（ P <0.01）。结论：蒙药 TGLG -1具有较好的降低高脂大鼠血脂和预防高脂血症的作用，其降脂作用可能与 TNF -α有关。%Objective:To probe into the adjustive effect of TGLG - 1 on the level of serum lipids and expression of TNF -α in the adipose tissues of hyperlipidemic rats. Methods:The model of hyperlipidemic rats was established with one - month high - lipid diet. Using full - automatic bio - chemical analyse instrument,the level of serum lipids,organ coefficient and the changes ofliver morphology in the rats in each group were determined 6 weeks after gastro - perfusion of TGLG - 1. The expres-sion of TNF - α in adipose tissues and the changes in the number of lipocytes were detected by immunohistochemical method. Results:The levels of serum total cholesterol(TC)and triglyceride(TG)in the rats in hyperlipidemia model group were signifi-cantly higher than those in the control group( P < 0. 01),with adipose degeneration significant in hepatic tissue,lipocytes en-larged and the number decreased,which suggested that the model was successful. The levels of TC and TG significantly dropped and the level of high - density lipoprotein(HDL - C)significantly rose( P < 0. 01)6 weeks after the treatment with TGLG - 1, with adipose degeneration alleviated in hepatic tissue. The results of immunohistochemistry showed that the expression of TNF -α in adipose tissue was more significant in hyperlipidemic model group than that in the other three groups( P < 0. 01),with the expression of TNF - α least significant in TGLG - 1 group( P < 0. 01). Conclusion:Mongolia drug TGLG - 1 could remarkably lower the serum lipid level in hyperlipidemic rats and prevent hyperlipidemia,whose hypolipidemic effect may be related to TNF - α.