细胞周期依赖性激酶抑制基因与脑梗死的关系

摘要

目的：探讨细胞周期依赖性激酶抑制基因（cyclin dependent kinase inhibitors ，CDKN2B）多态性及其与脑梗死发病的关系。方法：以rs3217992位点为遗传标记，采用聚合酶链式反应和限制性片段长度多态性（PCR －RFLP）方法检测180例脑梗死患者和120例对照者的基因型，用 Logistic回归分析基因多态性与脑梗死的关系。结果：脑梗死组 A 等位基因频率较对照组增高（χ2＝33．568， P＜0．001），脑梗死组 AG＋ AA 基因型频率较对照组增高（χ2＝28．755，P ＜ 0．001），Logistic回归分析显示 CDKN2B基因 rs3217992位点AG＋ AA 基因型是脑梗死发病的独立危险因素。结论：CDKN2B基因rs3217992位点多态性与脑梗死的发病可能有关，AG＋ AA 基因型为脑梗死患者发病的独立危险因素。%Objective :To investigate cyclin -dependent kinase inhibitor 2B(CDKN2B)polymorphism and its association with cerebral infarction .Methods :180 patients with cerebral infarction were recruited into this study ,and 120 healthy people were included as controls . SNP rs3217992 was used as the genetic marker .PCR -based restriction fragment length polymorphism analysis was applied to detect the genotype rs3217992 in the two groups ,and the association between CDKN2B polymorphism and cerebral infarction was analyzed using lo‐gistic regression .Results :The frequency of allele A and AG + AA genotype was significantly higher in cases than that in controls (χ2=33 . 568 ,P <0 .001)(χ2=28 .755 ,P <0 .001) .Logistic regression analysis suggested that AG + AA genotype in CDKN2B rs3217992 was the independent risk factor of cerebral infarction .Conclusion:CDKN2B polymorphism of rs3217992 is likely to contribute to the etiology of cerebral infarction .AG+ AA genotype may be the independent risk factor of cerebral infarction .