一氧化氮对神经细胞毒性作用的研究

摘要

Objective　To observe the neurotoxicity of nitric oxide. Method　We established the neurotoxicity model of rats cerebral primary culture neuron in vitro to observe the effect of L-Arg of different concentration on the neuron by using inveryted microscope. Results　 The action of L-Arg on neuron was correlated with its concentration. L-Arg of 40μM caused slight injury on neuron, but serious damage was found in the groups from 10-20μM and from 80 to 1280μM, when L-Arg was above 40μM, the net increase of LDH had positive correlation with the concentration of L-Arg(P＜0.01).Conclusion　The neuron is partially damaged when the concentration of L-Arg is 40μM, which is proper in the drug-protecting trial. When the concentration is above 40μM,neuronal mortality rate has linear correlation with the concentration of L-Arg.%目的观察一氧化氮对神经细胞的毒性作用。方法我们利用大鼠脑神经细胞体外原代培养NO毒性模型，借助倒置显微镜观察不同深度L-Arg对神经细胞作用。结果不同浓度L-Arg对神经细胞起不同作用。L-Arg浓度在40μM对神经细胞损伤较轻，介于10～20μM或80～1280μM之间对神经细胞损伤较大，L-Arg浓度>40μM后，LDH(乳酸脱氢酶)净增加率与L-Arg浓度的增加呈线性关系，具有统计学意义（P<0.01）结论 L-Arg浓度在40μM时部分神经细胞受损，此浓度为进行药物保护实验时L-Arg的最适浓度。L-Arg浓度>40μM后，细胞死亡率于L-Arg浓度增加呈线性关系。