番鸭小鹅瘟病直接荧光诊断试剂的研制

摘要

将抗番鸭GPV单抗腹水采用透析法标记异硫氰酸荧光素（FITC），制备成抗GPV荧光抗体，研制检测GPV抗原的直接免疫荧光诊断方法。结果显示GPV荧光抗体仅与GPV阳性的组织切片或细胞呈现特异性荧光，与番鸭细小病毒（MPV）、番鸭呼肠孤病毒（MDRV）、鸭副粘病毒（DPMV）、鸭病毒性肝炎病毒（DHV）和正常番鸭组织切片不反应；与间接荧光方法的符合率为92．9％。表明GPV荧光抗体具有较好的特异性、敏感性和准确性，可用于临床快速诊断番鸭小鹅瘟病。%Monoclonal antibody against goose parvovirus derived from muscovy duck was used to mark with fluorescein iso- thiocyanate by dialysis to manufacture fluorescent antibody in orger to prepare direct fluorescence assay （DFA） for gosling plague virus. The results showed that GPV fluorescent antibody only reacted with GPV-- positive--tissue slice or cell to pres- ent specific fluorescent, but not react with muscovy duck parvovirus （MPV）, muscovy duck reovirus（MDRV） .duck paramyxo- viruses （DPMV）,duck hepatitis virus（DHV） and normal tissue. The coincidence of DFA and with indirect fluorescence assay （IFA） was 92.9% for detecting GPV. The results above indicated that GPV fluorescent antibody had good specificity, sensi- tivity and accuracy so as to diagnose muscovy duck gosling plague.