In order to provide the basis for the scale, and standardization, industrialization production of Den- drobium huoshanense, research was taken on tissue rapid propagation of Dendrobium huoshanense , which uses the wild Dendrobium huoshanense seed as explants. The results showed that the most suitable medium for seed germination and protocorm induction was MS ＋ 6 - BA 0.1 mg / L ＋ NAA0.5 mg / L ＋ 20% potato juice ; the most suitable medium for protocorms continued metabolic reproduction and differentiation was 1/2MS ＋ 6 - BA 0.5 mg/ L ＋ NAA 0.1 mg/L, ＋20% potato juice;the most suitable medium for buds increase and multiply was 1/2MS ＋ 6 - BA 0.5 mg / L ＋ NAA0.5 mg / L ＋ 20% potato juice ; the most suitable medium for strong seedling seedling rooting was 1/2MS ＋ NAA0.5 mg/L ＋20% potato juice;the optimum temperature, light and time for acclimatization is at temperature of （24 ±2）℃, light： the last 3 days; the most suitable formula for plantlets transplant （1： 1： 1）. 6000Lx, time： 10days and open the cap was grass carbon root ＋ wood chips ＋ during Gravel%以野生霍山石斛种子为外植体，对霍山石斛组培快繁技术进行研究，旨在为霍山石斛规模化、标准化、产业化生产提供依据。结果表明，种子萌发与原球茎诱导最适培养基为MS＋6-BA0．1mg／L＋NAA0．5mg／L＋20％土豆汁；原球茎继代增殖与分化最适培养基为1／2MS＋6-BA0．5mg／L＋NAA0．1mg／L＋20％土豆汁；丛生芽增殖最适培养基为1／2MS＋6-BA0．5mg／L＋NAA0．5mg／L＋20％土豆汁；壮苗生根最适培养基为1／2MS＋NAA0．5mg／L＋20％土豆汁；炼苗最适温度、光照及时间：温度（24±2）℃，光照6000Lx，炼苗时间10d，后3d将瓶盖打开；试管苗移栽最适基质配方：草碳根＋碎木屑＋碎石（1：1：1）。
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