拟南芥中1个新snoRNA基因的鉴定

摘要

[目的] 鉴定拟南芥中1个新snoRNA基因.[方法] 运用生物信息学方法筛选拟南芥基因组序列,并对候选的基因序列结构、基因组织形式和功能进行分析.[结果] 在拟南芥基因组中发现snR95 box H/ACA snoRNA上游有一段序列具有典型box C/D snoRNA的保守组件和结构特征,并具有2段与rRNA互补、长度超过数10个核苷酸的反义序列,该基因下游的反义序列与snoRNA数据库中水稻Z270的下游反义序列一致,命名为box C/D snoRNA-AthZ270.[结论] 拟南芥Z270 snoRNA具有不同与一般snoRNA的功能.%[Objective] To identify a novel snoRNA gene in Arabidopsis thalianan. [Method] Genome sequence of Arabidopsis thalianan was screened by using bioinformatics methods, and the sequence structure, organization form and function of typical candidate gene were analyzed. [Result] The identified snR95 box H/ACA snoRNA had conservative component and structural features of box C/D snoRNA family, possessed two more than 10 nt long rRNA antisense elements. The result revealed that the novel snoRNA is a partial counterpart of the rice Z270, named box C/D snoRNA-AthZ270. [Conclusion] Z270 snoRNA in Arabidopsis thalianan has different function with common snoRNA.