花粉育性基因MS2的克隆与RNAi载体构建

摘要

[Objective]The aim was to study the function of MS2 gene. [Method] Cotton pollen fertility (MS2) gene cDNA fragment was cloned by RT-PCR;the senseMS2gene and antisense cDNAs of MS2 gene were ligated with the first intron of cotton chinase gene;the three fragments-fused sequence was identified and inserted into the plant expression vector of PBI121.[Result] The RNAi vector P35S12MSIn of MS2 gene was successfully constructed. [Conclusion]The vector created the conditions for genetic transformation in cotton sterile material .%[目的]研究MS2基因的功能.[方法]采用 RT-PCR克隆棉花花粉育性 (MS2)基因cDNA片段,将MS2反义基因、与陆地棉chinase基因第一个内含子和MS2正义基因 3个片段串联在一起 ,经鉴定后,插入到植物表达载体中PBI121中.[结果]成功构建了MS2基因的RNAi载体p35S12MSIn.[结论]该载体的构建为棉花不育材料的遗传转化创造了条件.