[目的]对按树基因组中葡萄糖-6-磷酸脱氢酶(G6PDH)进行全基因组分析与进化研究.[方法]利用巨桉(Eucalyptus grandsis)全基因组数据,采用BLAST等生物信息学软件分析桉树G6PDH的基因特性、蛋白序列、系统进化树以及启动子特征.[结果]桉树基因组内存在6个G6PDH基因,其编码蛋白中,1个为胞质型G6PDH,5个为质体型G6PDH,而且按树G6PDH均含有保守基序motif1、motif2、motif3、motif7、motif9和motif11.此外,桉树G6PDH启动子序列中均存在TATA框、增强子与涉及光应答、激素应答、胁迫应答等调控元件.[结论]可为下一步研究桉树G6PDH家族基因的分子功能提供参考.%[Objective] The aim of this study was to perform genome-wide analysis of glucose-6-phosphate dehydrogenase ( G6PDH) and reveal its evolution in Eucalyptus grandsis. [ Method] The gene character, protein sequence and phylogenetic tree of G6PDH gene were analyzed by BLAST and other bioinformatics software within Eucalyptus grandsis whole genome database. [ Result] Six G6PDH genes, including one cy-tomic type and five plastids, were detected in the E. Grandsis genome. All the G6PDHs had conserved motifs of motif 1, motif 2, motif 3, motif 7, motif 9 and motif 11. Furthermore, promoter sequences of all E. Grandsis G6PDH contained TATA box, enhancer, light-responsive, hormone-responsive and stress-responsive regulatory elements. [ Conclusion] This study provided reference for further revealing molecular function of E. Grandsis G6PDH gene family.
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