堆型艾美耳球虫cSZ1基因的克隆与分析

摘要

[目的]分析堆型艾美尔球虫cSZ1基因序列,为研制球虫疫苗提供候选抗原.[方法]根据堆型艾美尔球虫基因序列,设计合成引物,RT-PCR扩增堆型艾美尔球虫长春株cSZ1基因并测序.[结果]与GenBank中发表的堆型艾美尔球虫cSZ1基因序列相比,堆型艾美尔球虫长春株cSZ1基因发生了5个核苷酸变异,两者的核苷酸序列同源性为99.47％,两者编码区的核苷酸序列同源性为99.61％.堆型艾美尔球虫长春株cSZ1基因ORF内有2个变异位点.其中,A185G变异导致了所编码氨基酸由缬氨酸变为异亮氨酸.[结论]cSZ1基因编码蛋白可以作为球虫疫苗的候选抗原.%[Objective] To analyze Eimeria acervulina cSZl gene sequence and thus to find a candidate antigen for vaccine development. [ Meth od] According to the cSZl gene sequence of Eimeria acervulina published in GenBank,a pair of specific primers was designed. Then,the cSZX gene of Eimeria acervulina Changchun strain was amplified by RT-PCR and sequenced. [ Result ] Compared with the published cSZl gene se quence, five nucleotide mutation sites appeared in the cSZl gene of Eimeria acervulina Changchun strain. They had a nucleotide sequence homol ogy of 99.47% ,and their encoding region had a nucleotide sequence homology of 99.61%. Among these mutation sites,two appeared in cSZX gene ORF.and A185C mutation led to substitution of valine by isoleucine. [ Conclusion] Protein encoded by cSZX gene can be used as a candi date antigen for vaccine development.