[Objective ]The paper wag to study and analyze the genetic diversity of 48 endophytic bacteria isolated from Sophora alopecuroide with strong antagonistic effect against Fusarium oxysporum and Verticillium dhhliae, and carry out sequence measurement and phylogenetic a-nalysis on 10 representative strains. [Method] Using total DNA ERIC-PCR and 16S rRNA sequencing analysis method, a total of 48 endophytic bacteria were carried outt genetic diversity and phylogenetic analysis. [Result] ERIC-PCR fingerprint map showed that 48 endophytic bacteria were divided into 6 ERIC groups and two independence strain at Watson distance of 0.31. Representative strains were selected from each group to determine 16S rRNA gene for phylogenetic analysis, and the results showed that these strains belonged to Bacillus atrophaeus , Bacillus subtilis. Bacillus cereus, Bacillus megaterium, Bacillus pumilus and Serratia marcescens, respectively, [Conclusion ]It's obvious that the genetic diversity of 48strains of bacteriaf. Determination of 48 strains of bacteria on diversity and phylogenetic status could lay foundation for study on mechanism of F. oxysporum. And K dahliae, which could also provide new ways for biological control of diseases in cotton production.%[目的]研究了分离自苦豆子,对棉花枯萎病菌和黄萎病菌有较强拮抗作用的48株内生细菌进行遗传多样性,分析并对有代表性的10株菌进行序列测定和系统发育分析.[方法]采用总DNA ERIC-PCR方法和16S rRNA基因全序列分析方法对48株内生细茵进行了遗传多样性和系统发育分析.[结果]ERIC-PCR指纹图谱分析表明,在Watson距离为0.31时,48株菌被划分为6个ERIC群和2个独立成群的菌株,从每个群中挑选出代表性的菌株测定英16S rRNA基因,进行系统发育分析,结果表明这些菌株分别属于Bacillus atrohaeus、Bacillus subtilis、Bacillus cereus、Bacillus megaterium、Bacillus pumilus、Serratia marcescens.[结论]谊48株内生菌遗传多样性明显,通过对该48株菌的多样性和系统发育地位的确定,可以为研究棉花枯萎病菌和黄萎病菌的机制研究奠定基础,同时为棉花生产中病害的生物防治提供新的途径.
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