猪胎儿成纤维细胞APOE基因的差异表达研究

摘要

[目的]研究APOE基因在不同代数的猪胎儿成纤维细胞中的差异表达.[方法]收集正常传代生长的1、10、15、20、25、50代猪胎儿成纤维细胞,分别提取细胞总RNA.采用RT-PCR技术,检测不同代数猪胎儿成纤维细胞中APOE基因的表达.[结果]猪APOE基因在第1代猪胎儿成纤维细胞中表达水平最高,随着细胞代数的增加,表达童逐渐下降,到第50代表达童为最低.[结论]APOE基因在不同代数的猪胎儿成纤维细胞中有选择性表达的趋势.%[ Objective ] The aim was to study the differential expression of APOE gene in different generations of porcine fetal fibroblasts cells. [ Method] The first, tenth, fifteenth, twentieth, twenty-fifth, fiftieth generations of porcine fetal fibroblast cells, which were normally grown and passed, were collected before total RNA was extracted respectively. The expression of APOE gene in different generations of porcine fetal fibroblast cells was detected by RT-PCR technique. [ Result] The expression level of porcine APOE mRNA in the first generation of porcine fetal fibroblast cells was highest, and then it gradually decreased with the increase of cell generations and was lowest in the fiftieth generation. [ Conclusion ] The expression of APOE gene had the selective trend in different generations of porcine fetal fibroblast cells.