pH及浓度对次氯酸钠除藻效果的影响

摘要

[目的]研究次氯酸钠在不同浓度和不同pH条件下对羊角月牙藻的杀灭效果.[方法]以细胞数和叶绿素a作为检测项目,研究次氯酸钠在不同条件下对羊角月牙藻的杀灭效果以及叶绿素a褪色后的藻细胞活性.[结果]次氯酸钠浓度越高,叶绿素a的褪色效果越好,但细胞数减少量变化不明显.在3mg/L的NaClO条件下,pH为4.7的藻液中叶绿素a褪色率(62%)是pH为9.4(5%)的12倍.叶绿素a褪色率为95%和100%的藻液离心后重新接种到新鲜培养基中,藻细胞的数量呈逐渐下降趋势.[结论]pH越低,次氯酸钠的除藻效果越好.叶绿素a褪色后藻细胞失去了生长繁殖的能力.通过调节pH和NaClO浓度相结合的方法可有效抑制藻类生长,控制藻类的爆发.%[ Objective ] To study the influence of different concentrations and different pH value on killing the Selenastrum caprlcornurum and the discolourization of chlorophyll a with sodium hypochlorite. [ Method] The effect of S. caprlcornurum killed by sodium hypochlorite in different conditions and the activity of discolored algae cell was studied through measuring the cells and the contents of chlorophyll a. [ Result] The results showed that higher concentrations favors discolourization of chlorophyll a, but algae removal was not evident. The discolored rate of chlorophyll a at pH 4.7 was 12 times higher than that at pH 9.4 under the concentrations of 3 mg/L of sodium hypochlorite. The amount of the algae was winded down when the inoculums with different discolored rate (95％ and 100％ ) was centrifugalize to re-inoculated into the fresh SE media. [ Conclusion] The discolored rate of chlorophyll a by sodium hypochlorite was higher with the lower pH value. The reproductive capacity of S. caprlcornurum was lost without the chlorophyll a. So the method using sodium hypochlorite by adjusting the pH value to prevent from the algae outbreak was feasible.