狼爪瓦松全草粗提物体外抑菌作用研究

摘要

[ Objective ] To study antibacterial activities of crude extracts from the whole herb of Orostachys cartilaginous A. Bor in vitro. [ Method] liquid -liquid extraction technique was applied to isolate active ingredients from 0.75 acetone extracts for preparing chloroform,ethyl acetate ,normal butanol and water phases. The antibacterial activities of extraction phases on 4 tested strains in vitro were investigated using the filter paper method and double dilution method. [ Result ] Extraction phases from 0.75 acetone extracts had antimicrobial activities against Staphyloccocus aureus Rosenbach,Bacillus subtilis( Ehrenberg) Cohn,Escherichia coli and Proteus vulgaris in varying degrees. Chloroform and ethyl acetate extraction phases were provided with strong antibacterial activities against Staphylocoaus aureus with minimal inhibitory concentration at 1.25 mg/ml. [ Conclusion] Active ingredients of Orostachys cartilaginous A. Bor plant were mainly existed in chloroform and ethyl acetate phases,which could provide a scientific basis for developing antibacterial agents from Orostachys cartilaginous A. Bor.%[目的]研究狼爪瓦松(Orostachys cartilaginous A.Bor)全草粗提取物体外抑菌作用.[方法]采用液-液萃取法对狼爪瓦松的体积分数为0.75的丙酮提取液的活性成分进行初步分离,制备氯仿、乙酸乙酯、正丁醇及水相萃取物.采用滤纸片法和2倍连续稀释法测定了各相萃取物对4种供试菌的体外抑菌效果.[结果]狼爪瓦松全草体积分数为0.75的丙酮提取液的各萃取相对金黄色葡萄球菌、枯草芽孢杆菌、大肠杆菌和普通变形杆菌有不同程度的抑菌活性,其中氯仿萃取相、乙酸乙酯萃取相对金黄色葡萄球菌显示了较好的抑菌效果,最低抑菌浓度均为1.25 mg/ml.[结论]中药狼爪瓦松全草的抑菌活性成分主要存在于氯仿萃取相和乙酸乙酯萃取相中,为狼爪瓦松抑菌药物的开发提供了理论依据.