[目的]利用食品废水培养枯草芽孢杆菌(Bacillus subtilis)ASI-296产絮凝剂吸附cu<'2+>.[方法]以味精废水和啤酒废水作为替代培养基培养枯草芽孢杆菌ASI-296制备含γ-聚谷氨酸的絮凝剂,通过正交试验确定其最佳培养条件,对枯草芽孢杆菌产的絮凝剂进行了明胶-戊二醛交联固定处理,并对Cu<'2+>进行吸附和解析研究.[结果]培养枯草芽孢杆菌ASI-296产絮凝剂的最佳条件为:pH为7,培养时间为48 h,啤酒废水与味精废水的体积比为15.红外光谱分析结果显示,絮凝剂主要由γ-聚谷氨酸和多糖组成.固定后的絮凝剂最佳Cu<'2+>吸附条件为:最佳吸附pH为5,絮凝剂投加量为0.6 g,温度为40℃,Cu<'2+>初始浓度为30 ms/200 ml,最大Cu<'2+>吸附容量为4.1 mg/g.最佳Cu<'2+>解析条件为:pH为2,最大解析率为81%.[结论]利用味精废水和啤酒废水作为主要原料,使培养枯草芽孢杆菌AS1-296产絮凝剂更具有经济和实用价值,真正实现了"以废治污".%[Objective] The Bacillus subtilis-AS1-296 was cultured with wastewater from the food-producing for the formation of the flocculant adsorbing Cu2+. [ Method]The Bacillus subtilis-AS1-296 was cultured with the wastewater from glutamate and brewery-producing instead of its medium for the preparation of y-polyglutamic acid containing flocculant. The culture condition for the flocculant production from Bacillus subtilis was optimized through the orthogonal experiment. The flocculant was fixed with gelatin-glutaraldehyde and its adsorption and analytical capacity to Cu2+ was researched. [ Result]The best culture condition of AS1-296 for flocculant production was the pH value was 7, the culture time was 48 hours and the ratio of beer to glutamate wastewater in volume was 15: 1. The inspection result of infrared spectroscopy showed that the flocculation consisted mainly of y-polyglutamic acid and polysaccharide. Its best adsorption condition after fixed was: the pH value was 5, the flocculant dosage was 0. 6 g, the temperature was 40 ℃ and the initial concentration of Cu2 + was 30 mg/200 ml, under which, the maximum adsorption capacity of Cu2+ was 4. 1 mg/g; and its best analytical condition was s for the pH value was 2 and the maximum analytical rate was 81%. [ Conclusion] The use of glutamate and brewery wastewater being taken as main raw material for the Bacillus subtilis AS1-296 culturing for the flocculant production would be with more economic and practical value so that it could be realized that the waste material be reused for the control of its pollution.
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