云南鬼灯檠属植物中岩白菜素含量比较

摘要

[目的]比较云南鬼灯檠属植物不同种及产地样本的岩白菜素含量.[方法]采用HPLC 测定云南鬼灯檠属植物不同种及产地样本的岩白菜素含量.色谱柱为Agilent Zorbax XDB-C.柱(4.6mm×150mm,5μm),流动相为乙腈(A)-0.2%磷酸水溶液(B)梯度洗脱(0-10 min,0%-20%A,100%-80%B),流速为1.0 ml/min,柱温为30℃,进样量为10μl,检测波长275 nm.[结果]岩白菜素在3～48μg/ml 之间与吸光度值呈良好的线性关系,相关系数为0.999 6.4个种及变种的鬼灯檠属植物样本岩白菜素含量以西南鬼灯檠平均含量最高为5.59%,七叶鬼灯檠平均含量最低为3.45%.[结论]鬼灯檠属植物不同种之间岩白菜素含量不同,同种不同居群之间的岩白菜素含量变化也明显.%[ Objective ] To compare the content of bergenin in the Yunnan genus Rodgersia with different species and growing areas. [Method]The content of bergenin in the Yunnan genus Rodgersia with different species and growing areas was determined by HPIC. The chromatographic conditions were as follows： column of Agilent Zorbax XDB-C18(4.6 mm ×l50 mm, 5 μm), mobile phase of acetonitrile(A) and 0.02％ phosphoric acid solution ( B ), gradient elution ( 0-10 min,0％ - 20％ A, 100％ - 80％ B ), flow rate of 1 ml/min, column temperature of 30 ℃, sampling volume of 10 μl and detection wavelength of 275 nm. [ Result] Bergenin showed a good linear relationship with absorbance in the range of 3 to 48 μg/ml ( r=0. 999 6). Bergenin content was the highest in Rodgersia sambucifolia Hemsl. with 5.59％, and the lowest in Rodgersia aesculifolia Batalin with 3.45％. [ Conclusion ] Bergenin content in the genus Rodgersia was various in different species, and there were obvious differences among different populations from the same species.