Cu2+、Cd2+、Cr6+胁迫对斑马鱼抗氧化酶活性的影响

摘要

[ Objective ] The aim was to study the effect of Cu2+, Cd2+ and Cr6+ on the anti-oxidative enzyme activities in Danio rerio, and discuss the toxicity effect of heavy metal on Danio rerio. [ Method] Danio rerio was viewed as model organism, semi-static toxicity test was used in domestication and experiments of Danio rerio, the effects of Cu2+ , Cd2+ and Cr6+ on superoxide dismutase (SOD), peroxiclase (POD) and catalase (CAT) of it were discussed. [ Result] At 1 d, effects of Cu2+ stress on SOD in Danio rerio was induction effect, and the stress on POD was inhibition-induction-inhibition effect, and stress on CAT was induction effect. At 7 d, effects of Cu2+ stress on SOD in Danio rerio was induction effect, and stress on POD was inhibition effect, and stress on CAT was inhibition effect. At 1 and 7 d, effects of Cd2+ stress on SOD in Danio rerio were all induction effect, and stress on POD and CAT were all inhibition effect. Moreover, the enzyme activities were changed markedly significantly at from 1 to 7d. [ Conclusion] Cu2+ , Cd2+ and Cr6+ single stress on the anti-oxidative enzyme activities in Danio rerio were changed markedly. In short time and low-concentration exposure, Cr6+ toxicity was the most significantly; in short time and high-concentration exposure, Cd2+ toxicity was the most the most significantly; in long time exposure, Cu2+ toxicity was the most significantly.The study could provide references for heavy metal pollution on the aquatic organism toxicity mechanism.%[目的]研究Cu、Cd、Cr对斑马鱼抗氧化酶活性影响,探讨重金属对斑马鱼毒性大小.[方法]以斑马鱼为模式生物,采用半静态法对斑马鱼进行驯养和试验,测定Cu、Cd和Cr对超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性影响.[结果]Cu暴露1d胁迫斑马鱼SOD和CAT为诱导效应,胁迫POD为抑制-诱导-抑制效应,Cu暴露7 d胁迫斑马鱼SOD为诱导效应,胁迫POD和CAT为抑制效应;Cu和Cr暴露1 d和7 d胁迫斑马鱼SOD和CAT均为诱导效应,胁迫POD为抑制效应.且暴露1 d和暴露7 d酶活性变化均显著.[结论]Cu、Cd、Cr单一胁迫斑马鱼抗氧化系统酶活性变化显著,在短时间和低浓度暴露下,Cr毒性作用最显著;在短时间中、高浓度暴露下,Cu毒性作用最显著;长时间暴露时,Cu毒性作用最强,可为重金属污染对水生生物毒性机理研究提供参考.