Algae-lysing bacteria Al has good control effect on the moss through its extracellular secretion. In order to further study its control mechanism,the single-factor tests of temperature and pH value, the activated carbon adsorption test,the organic solvent extraction test and the crude extract of active substance test were carried out to explore the characteristics of active substances. The results showed that the algae-lysing active ingredient of Al strain had strong thermal stability, which still had good ulgae-lysing effect after being treated at 121 ℃ ;when the pH values of fermentation liquid were adjusted to 2.0 and 4.0 respectively, the active substances lost their activities, but their control ability against moss would be enhanced under the alkaline conditions; the active substances could not be adsorbed by activated carbon;the algae-lysing active substances showed strong hydrophilic ability when they were extracted by ethyl acetate, petroleum ether and chloroform. Therefore ,it could be speculated that the active substances belonged to carbohydrates. The crude extract results of algae-lysing active substances showed that the active substances secreted by Al strain were composed by a variety of algae-lysing active substances.%溶藻细菌Al是通过分泌胞外物质对青苔起到防除作用的.为进一步研究其防除机制,通过温度和pH值的单因素试验,活性炭吸附、有机溶剂萃取以及活性物质粗提等试验探讨活性物质的性质.研究结果表明:Al菌株溶藻活性成分具有很强的热稳定性,经过121℃处理后仍有很好的溶藻效果;发酵液的pH值分别调至2.0和4.0时,活性物质失活,而中碱性条件下活性物质防除青苔能力会增强;该活性物质不能被活性炭吸附;有机溶剂乙酸乙酯、石油醚和氯仿萃取后,该溶藻活性物质表现出强亲水性,由此可以初步推测活性物质属于糖类.溶藻活性成分粗提结果表明,Al菌株分泌的活性成分应由多种溶藻活性物质组成.
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