苹果近红外光谱采集影响因素研究

摘要

[目的]对不同影响因素条件近红外光谱信息的准确性进行研究,优化嘎啦苹果近红外光谱采集试验条件. [方法]以嘎啦苹果为试材,使用ASD公司的FieldSpec3光谱仪在常温条件下进行近红外光谱采集,探索了环境杂散光、仪器稳定性、不同测距、不同色差、不同部位、不同货架期等条件对采集光谱的影响. [结果] 杂散光对近红外光谱的可见光区域有明显影响;固定光谱阵列检测器型光谱仪器在9 h内重复测量的稳定性高;裸光纤在除0 mm外的2.5～12.5 mm测距上吸光度保持重复稳定;同一苹果的不同色差对光谱的影响在可接受区间内;苹果赤道面上的光谱稳定性较果柄和果鄂部好;常温条件下,不同货架期对苹果样品的近红外光谱产生显著影响. [结论]该研究可为苹果近红外研究人员和分析工作者提供参考.%[ Objective] The aim was to optimize experimental conditions of Gala apple NIR ( Near Infrared) spectroscopy acquisition through studying on the accuracy of NIR spectroscopy information under different influence factors. [ Method] Gala apple was taken as the experimental material, and FieldSpec3 spectrometer (Analytical Spectral Device Company, America) was employed to acquire the NIR spectroscopy at room temperature. Then, the effects of different conditions including the environmental stray light, instrument stability, different distance, different chromatic aberration, different sites and different shelf-life on spectroscopy acquisition were investigated. [ Result] The stray light had a significant effect on the visible light region of NIR spectroscopy; FieldSpec3 spectrometer had higher stability in 9 h; the measure distance of 2.5 - 12.5 mm could obtain good repeatability of absorbance; the effects of different chromatic aberration of the same apple on spectra was acceptable; the spectral stability of equatorial plane of the apple was better than those of the bottom and stalk; at room temperature; different shelf-life could make significant influence to NIR spectroscopy of the apple simple. [ Conclusion ] This study will provide reference for the researchers and analysis workers of apple NIR spectroscopy.