[ Objective ] The aim was to study on the effects of heavy metal stress on the growth curves of Escherichia coli and Bacillus subtili.[ Method ] Using traditional culture method, Escherichia coli and Bacillus subtili were cultured under heavy metal stress including Cu2+ , Hg2 +,Pb2 + , Cd2 + and Cr6+ in different concentrations. Then the growth curves of the bacteria were determined to investigate the effects of exogenous heavy metals on the growth of the two kinds of bacteria. [ Result] The proliferation of the two bacteria was inhibited at high concentrations of Hg2+ and Cd2+ respectively, and G+ is more sensitive to them than G-; when the heavy metal concentration was ＞ 50 mg/L, the toxicity of the five kinds of heavy metals on the two bacteria was Hg2+ ＞ Cd2+ ＞ Cu2+ ＞ Cr6+ ≈Pb2+. [ Conclusion] The research will provide a basis to explore the effects of heavy metal on environment and ecological system.%[目的]研究重金属胁迫培养对2种细菌生长曲线的影响.[方法]以大肠杆菌和枯草芽孢杆菌2种典型细菌为试验材料,采用传统培养方法,选择5种重金属离子Cu2+、Hg2+、Pb2+、Cd2+、Cr6+在不同浓度下对其进行胁迫培养,通过测定2种细菌的生长曲线,研究外源性重金属对2种细菌生长的影响.[结果]Hg2+、Cd2+毒性较强,2种细菌的增殖在高浓度时受到抑制,G+比G-更为敏感;当重金属浓度＞50 mg/L后,5种重金属的对2种细菌的毒性顺序为Hg2+＞Cd2+＞Cu2+＞Cr6+≈Pb2+.[结论]该研究结果为进一步研究重金属对环境及生态系统的影响奠定基础.
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